Part:BBa_K1645999:Experience

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Applications of BBa_K1645999

We used Imaging Flow Cytometry to characterise this part, as it allowed us to visualise the expression of both red and green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB overnight. The cells were fixed and diluted 10 times in 10% formalin buffer before use in Imaging FLow Cytometry, where they were excited by a laser optimised for reading at 488nm. In order to control for signal leakage between channels, BBa_I20260 and BBa_J04450 were used as single colour controls. This intensity plot of both colours was created.

The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. Link title

User Reviews

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